Rapid Agrobacterium-mediated genetic rice transformation method using liquid media
نویسندگان
چکیده
منابع مشابه
Optimization of Agrobacterium-Mediated Genetic Transformation in indica Rice
This experiment was conducted during the period from August 2004 to April 2005 in the Biotechnology & Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh. In this experiment, an efficient and reproducible protocol for the production of transgenic rice plants was developed by inoculating mature embryo of three indica ric...
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and the bar gene was transmitted through both male and female gametes and expressed in T1 progeny. Although Agrobacterium tumefaciens has been successfully used Microprojectile bombardment and gene transfer by to transfer genes to a wide range of plant species, it has received litA. tumefaciens are currently the two most common tle attention for transformation of forage grasses. Therefore, the ...
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Agrobacterium tumefaciens is a Gram-negative soil phytopathogenic bacterium that causes the crown gall disease of dicotyledonous plants, which is characterized by a tumorous phenotype. It induces the tumor by transferring a segment of its Ti plasmid DNA (transferred DNA, or T-DNA) into the host genome and genetically transforming the host. One century has past after A. tumefaciens was firstly i...
متن کاملGenetic Transformation of Oat Mediated by Agrobacterium is enhanced with Sonication and Vacuum Infiltration
Background: Oat (Avena sativa) with high nutritive value and fiber content is used as the main food grain in many countries for human diet as well as animal feed. Recently, it became difficult to transfer new genes through the conventional breeding due to the lack of desirable traits. Objectives: The current study aimed at achieving a st...
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While Agrobacterium-mediated transformation (AMT) remains the most widely used technique for gene transfer in plants, interest exists for the use of non-Agrobacterium gene delivery systems due to freedom-to-operate issues that remain with AMT across several jurisdictions. In addition, the plant pathogenic mode of action of Agrobacterium tumefaciens significantly increases the costs to passage e...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2013
ISSN: 1229-2818
DOI: 10.5010/jpb.2013.40.1.037